The evaluation of botulism begins with a medical history and physical examination.

Physical findings in a person with botulism may include:

• Muscle weakness
• Signs of dehydration
• Disconjugate eyes
• Wound infection

Testing is required to confirm the diagnosis of botulism.

Tests that may be used to evaluate botulism include:

• Wound cultures
• Blood tests to detect botulism
• Stool cultures to detect botulism
• Chest x-ray
• Pulse oximetry