The evaluation of acanthamoeba infection begins with a history and physical examination.

Physical findings in those with acanthamoeba infection may include:

• Cloudy cornea
• Balance difficulty
• Confusion
• Fever
• Stiff neck

Tests are necessary to make the diagnosis.

Tests for acanthamoeba infection may include:

• CT scanning of the brain
• Lumbar puncture
• Eye scrapings may be obtained for culture and stains